NBIMCC maintains microbiological objects preserved by freeze-drying and/or freezing in liquid nitrogen. Periodic checks for viability, quality control, revitalization and conservation of samples are carried out.

1. Types of deposit.

1.1. Open deposit.

The NBIMCC accepts microbiological samples of interest for the research investigations and industry, which are or will be cited in the literature. Information about the strains will be published in the catalogue. These cultures may be provided to third parties. Thus the biological material will be available to the international scientific community in both academic and industrial institutions.

The deposit in the public collection is carried out in accordance with the Protocol of Nagoya and the Convention on Biological Diversity.

1.2. Patent deposit.

NBIMCC accepts microbiological samples for patent purpose in accordance with the requirements of the Budapest treaty on the international recognition of the deposit of microorganisms for the purposes of patent procedure (BT). NBIMCC is responsible for viability and purity of the stored cultures for a period of not less than 30 years. The deposit will be recognized internationally. Information about the strains will not be published in the catalogue and they will not be available to third parties. The material and all information concerning it will be handled strictly confidentially. Samples will be only distributed within the strict restraints of the Budapest Treaty.

1.3. Safe deposit.

NBIMCC accepts for storage microbiological samples of interest to science, for a certain time, pursuant to a contract for safe deposit. Information about the strains will not be published in the catalogue and they will not be available to third parties. This type of deposit is used for Bulgarian customers or joint-stock companies having branch in Bulgaria registered in the Commercial register and register of NPLE.

2. General requirements.

2.1. NBIMCC accepts for deposit bacteria, actinomycetes, yeasts, fungi, plasmid-bearing bacteria, animal and plant viruses, and animal cell lines. This activity is part of the biological diversity preservation.

2.2. NBIMCC accepts for deposit only microbiological objects belonging to risk group 1 and 2.

2.3. The depositor must provide sufficient information about the biological material to draw up a proper risk assessment.

2.4. The deposited material must be: a pure culture for open deposit; a pure culture or defined as mixed one (up to 3 strains) for patent and safe deposit.

2.5. The deposited material can be preserved by cryogenic or lyophilic conservation without significant change.

3. Deposit procedure.

3.1. Users of the service can request for deposit in the NBIMCC by e-mail (,

3.2. Users can complete and send an Accession form for microorganisms for open and safe deposits. For the purposes of patent deposit users can complete and send an Accession form (BP1).

3.3. On request, NBIMCC sends accession forms for deposit of the other microbiological groups.

3.4. NBIMCC assesses whether will accept for deposit the strain/line, depending on its characteristics and cultivation conditions and sends a confirmation letter. NBIMCC may refuse a deposit in the case of incomplete documentation or impossibility to maintain the culture.

3.5. NBIMCC sends a Microbiological material deposit agreement to be signed by the depositor for deposit in the public collection.

3.6. The depositor dispatches the biological material according to point 4.

3.7. After proper preservation and viability check NBIMCC sends a deposit certificate.

3.8. For patent deposit NBIMCC sends international forms BP4 (receipt of the original deposit) and BP9 (viability statement).

4. Requirements for deposit of biological material.

4.1. Depositor can provide:

4.1.1. Microorganisms:

     Actively growing culture (preferable form):

-     open and patent deposit 3 plates with single colonies;

-     safe deposit 3-5 slants with compact growth.

     Freeze-dried culture:

-     open deposit not less than 15 samples;

-     patent deposit not less than 10 samples;

-     safe deposit not more than 20 samples.

     Frozen culture in cryotubes:

-     open and patent deposit this form is not acceptable;

-     safe deposit not more than 20 samples.

4.1.2. Animal cell lines frozen culture in cryotubes:

-     open deposit not less than 6 samples;

-     patent deposit not less than 12 samples;

-     safe deposit not more than 20 samples.

4.1.3. Animal viruses freeze-dried or frozen in cryotubes:

-     open and patent deposit not less than 20 samples;

-     safe deposit not more than 20 samples.

4.1.4. Plant viruses 5 g fresh leaf material with pronounced symptoms.

4.2. Biological material shall be provided in person or sent via courier service with appropriate packaging (e.g. dry ice, liquid nitrogen if necessary).

5. Schedule of fees for deposit services.

5.1. Open deposit no charges.

5.2. Patent deposit


1200 BGN

     Issurance of a viability statement (rule 10.2 of BT)

120 BGN

     Furnishing of a sample (rule 11 of BT)

120 BGN

5.3. Safe deposit an agreement shall be concluded with the depositor.

6. For further details please contact us on email: