Journal
of Culture Collections
Volume 3, 2000-2002
SEROLOGICAL AND ELECTROPHORETIC
CHARACTERIZATOIN
OF THE NECROTIC STRAIN
CMV-NB OF CUCUMBER MOSAIC VIRUS
Angela Yordanova1*,
Dimitrinka Hristova2 and Elisaveta Stoimenova3
1National Bank for Industrial Microorganisms and
Cell Cultures, 1113 Sofia, P.O.Box 239, Bulgaria;
2Plant Protection Institute, 2230 Kostinbrod, Bulgaria;
3Institute of Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
Summary
Serological and electrophoretic properties of
Bulgarian isolate CMV-NB causing tomato fruit necrosis were investigated. Its
serogroup affiliation was studied by means of indirect ELISA with polyclonal
and monoclonal antibodies as well as by immunodiffusion and
immunoelectrophoresis with sera against subgroups I and II of CMV. Strain NB belongs to subgroup I but distinguishes
from the other tested strains from this group in counter and rocket
immunoelectrophoresis especially. The electrophoretic mobility of capsid
protein and whole virions of NB was also compared with those of other virus
strains. Strain NB was characterized with very bigger mobility of
virus particles in electric field. It explained differences established in
immunoelectrophoretic analysis. The obtained results supposed presence of
mutations in CMV-NB, which leaded to increasing negative surface charges of
virions.
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