Journal of Culture
Collections
Volume 3, 2000-2002
Influence of the growth conditions on the
resistance of Saccharomyces cerevisiae,
strain NBIMCC 181, by freeze-drying
National Bank for Industrial Microorganisms and
Cell Cultures, 1113
Experiments
were carried out for determination the influence of the growth conditions on
the resistance of brewery yeast Saccharomyces cerevisiae,
strain 181, to freeze-drying. Growth curves were studied in parallel with the
viability of the strain by three variants of cultivation – in a liquid broth,
in a solid agar medium and in combination of liquid and solid agar nutrition
medium. Better preservation of the cell suspensions obtained in agar medium was
determined, where the maximum value was about 8.3‑8.7 % after
freeze-drying preservation at the 64th and 104th hour of
cultivation, whereas in a liquid broth it was 0.28–0.25 % between the 18th
and 24th hours. We propose a combinated two-step method for
consecutive growing in liquid and agar medium, by which viability of
13.2 % from the yeast cells after lyophilization was reached.