Journal of Culture Collections

Volume 3, 2000-2002

AN OPTIMISED METHOD FOR INVESTIGATION OF THE YEAST

VIABILITY BY MEANS OF FLUORESCENT MICROSCOPY

Maria Nikolova¹*, Irena Savova¹ and Minko Marinov²

¹National Bank for Industrial Microorganisms and Cell Cultures, 1113 Sofia, P.O.Box 239, Bulgaria;

²The Sofia University, Biological Faculty, Department of Engineering Biology,

8 “Dragan Tzankov” st., 1421 Sofia, Bulgaria

Summary

Based on the fluorescent microscopy the viability of a number of yeast strains was investigated. A double staining procedure with fluorescein diacetate and ethidium bromide was carried out. This method is fast, sensitive and very suitable for determination of yeast viability. It can be applied for ascomycetes as well as for basidiomycetes and imperfect fungi.

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